Double Digest Protocol with Standard Restriction Enzymes.Optimizing Restriction Endonuclease Reactions.

NotI-HF® has the same specificity of NotI (NEB #R0189), but it has been engineered for reduced star activity.

One unit is defined as the amount of enzyme required to digest 1 μg of pBC4 DNA in 1 hour at 37☌ in a total reaction volume of 50 μl. The following reagents are supplied with this product: NEB # coli strain that carries the cloned and modified NotI gene from Nocardia otitidis-caviarum (ATCC 14630) This product is related to the following categories: Restriction Endonucleases: N-O, High-Fidelity (HF®) Restriction Endonucleases Products, Time-Saver Qualified Restriction Enzymes Products, This product can be used in the following applications: Fast Cloning: Accelerate your cloning workflows with reagents from NEB, Restriction Enzyme Digestion Reagents Supplied Reagents Supplied The resolution isĪt the single nucleotide level. Is determined by capillary electrophoresis and peak analysis. Restriction Enzyme Competitor Study: Nuclease ContaminationĮcoRI, NotI, and BamHI from multiple suppliers were tested in reactionsĬontaining a fluorescent labeled single stranded, double stranded blunt,ģ’overhang or 5’ overhang containing oligonucleotides. Of our HF restriction enzymes are shown below. Examples of nuclease contamination studies for some NEB extensively performs quality controls on all standard and high-fidelity

Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. HF enzymes also exhibit dramatically reduced star activity. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. Other Products You May Be Interested In.Restriction Enzyme Digest Problem: Too Many DNA Bands.Why is My Restriction Enzyme Not Cutting DNA?.Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel.Restriction Enzyme Digest Protocol: Cutting Close to DNA End.TIME-SAVER ™ Protocol for Restriction Enzyme Digests.Reduce Star Activity with High-Fidelity Restriction Enzymes.

Neb noti hf